Algipore® Stimulates Osteblast Differentiation in Adipose Derived Stem Cells

ALGIPORE® STIMULATES OSTEBLAST DIFFERENTIATION IN ADIPOSE DERIVED STEM CELLS Brunelli G, Carinci F, Girardi A, Palmieri A, Caccianiga AGL, Gigante A, Sollazzo V. Advances in bone grafting are progressing with the evolution of biomaterials that permit the in corporation of osteoinductive and osteogenic proteins into osteoconductive composite scaffolds. Algipore®, a hydroxyapatite ceramic obtained from red alga, is largely employed as scaffolds in bone regeneration. For this reason, we studied if and how Algipore® can induce osteoblast differentiation in stem cells derived from adipose tissue, measuring the expression levels of bone related genes and mesenchymal stem cells marker by real time RT-PCR. The obtained results demonstrated that Algipore® enhances differentiation and deposition of matrix in stem cells by the activation of osteoblast and related genes FOSL1 , COL1A1, SPP1, SP7 and ALPL. Eur J Inflamm 2012;10 (S1);1-4 AN IN VITRO STUDY ABOUT THE ALLOGRO® EFFECT ON ADIPOSE DERIVED STEM CELLS AND HUMAN OSTEOBLAST: A COMPARATIVE STUDY. Brunelli G, Carinci F, Girardi A, Palmieri A, Caccianiga AGL, Gigante A, Sollazzo V. The reconstruction of bony defects is still a big concern for the oral surgeon. Autogenous bone is a good candidate for reconstruction, but it requires a second surgical procedure at a different site, involving additional time, extra cost, and potential for problems such as infection. At present, many bone-grafting products have been introduced. Allogro® (Ceramed, Lakewood, CO) is a demineralized freeze-dried bone allograft, useful as scaffold to fill bone defects and to restore bone loss in orthopedic and maxillofacial surgery. The present study shows that Allogro® leads to osteoblast phenotype expression and extracellular matrix deposition in ADSCs by the activation of osteoblast related genes SP7, COL1A1 and SPP1 and the under-expression of ENG. Eur J Inflamm 2012;10 (S1);5-9. AN IN VITRO STUDY ABOUT THE OSTEINDUCTIVE EFFECT OF BIO-OSS ON ADIPOSE DERIVED STEM CELLS AND HUMAN OSTEOBLAST: A COMPARATIVE STUDY Brunelli G, Carinci F, Perrotti V, Ricci L, Piattelli A, Girardi A, Palmieri A, Sollazzo V. The aim of the present study was to investigate how Bio-Oss® can induce osteoblast differentiation in adipose derived stem cells (ADSCs) by analyzing the expression levels of bone related genes and mesenchymal stem cells ADSCs were isolated by enzymatic digestion and centrifugation of adipose tissue and treated with Bio-Oss for 15 and 30 days. Gene expression analysis was performed by using Real Time Polymerase Chain Reaction. Our results showed that Bio-Oss is conducive to osteoblast and osteoclast, by activating bone related genes as SPP1, FOSL1, RUNX2, COL1A1 and SP7. Eur J Inflamm 2012;10 (S1);11-15.